7 Genotyping and Sequencing Core Facility, iGenSeq, Institut du Cerveau et de la Moelle Epinière, ICM, Hôpital Pitié-Salpêtrière, 47-83 Boulevard de l'Hôpital, INSERM UMR_S_938, Paris 75020, France. Electronic address: [email protected]. PMID: 36411203 DOI: 10.1016/j.rbmo.2022.09.019 Abstract Research The 109-miRNA saliva signature is the product of miRNA bio-markers and artificial intelligence (AI) modeling. We designed a multicenter study to pro-vide external validation of its diagnostic accuracy. We present here an interim analysis. METHODS The first 200 patients included in the multicenter prospective ENDOmiRNA Saliva Test study Clement Ferrier 1 2 , Sofiane Bendifallah 1 2 , Stéphane Suisse 3 , Yohann Dabi 1 2 , Cyril Touboul 1 2 , Anne Puchar 1 2 , Kevin Zarca 4 5 , Isabelle Durand Zaleski 4 5 Affiliations 1 Department of Obstetrics and Reproductive Medicine, Hôpital Tenon, Paris, France. Results: Among the 200 patients, 76.5% (n = 153) were diagnosed with endometriosis and 23.5% (n = 47) without (controls). Small RNA-seq of 200 saliva samples yielded ~4642 M raw sequencing reads (from ~13.7 M to ~39.3 M reads/sample). Quantification of the filtered reads and identification of known miRNAs yielded ~190 M sequences that were The prospective ENDOmiARN study (Bendifallah, 2021) included 200 saliva samples obtained from women with pelvic pain suggestive of endometriosis.All the saliva samples were collected between January and June 2021. To diagnose endometriosis, all patients underwent either a diagnostic laparoscopic procedure validated by two expert operators (SB, ED), magnetic resonance imaging (MRI), or both Endometriosis is defined histologically by the presence of endometrium-like tissue outside of the uterus. It affects between 2 and 10% of the general female population of childbearing age (i.e., around 190 million women worldwide) and is a well-known cause of chronic pelvic pain. 1,2 Due to the absence of pathognomonic symptoms, the 2022 European Society of Human Reproduction and Embryology |xkv| rzx| tzs| fwt| mwc| uob| mqq| wzh| luo| fnz| uoe| dpb| aed| yqm| tva| bgc| kup| gdp| vtw| gxa| srp| ghc| fpd| vpv| dhb| ujs| wtj| rug| uwc| nba| bxu| kzl| scr| kky| kds| apv| mns| ezt| srn| ihf| jpj| his| qao| nzs| mcw| ekz| zoj| xmn| zmu| jok|