Part Numbers: E2000. Colorimetric Detection of β-Gal Activity. Safe, non-isotopic assay. Can be used in 96-well plate format. Reporter Lysis Buffer allows luciferase, CAT and β-gal assays to be performed from the same cell extract. Size. 10ml. Catalog number selected: E2000. $ 205.00. Product Description. The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells. Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay. were measured by dual-luciferase Reporter Assay kit (Promega, Madison, WI, USA) at 48 h post-transfection. 2.7. Cell counting kit-8 (CCK-8) assay In brief, 10 lL CCK-8 (Solarbio, Beijing, China) was added into 100 lL cell culture supernatant of each well and incubated at 37°C for 1 h. Subsequently, the cell viability was analyzed by measuring Beta-Glo® Reagent was added to yeast grown to an A 600 of 0.1 or to standing phase yeast (A 600 >1.0) diluted to an A 600 of 0.1 and incubated for 60 minutes. β-galactosidase activity was measured using a luminometer and represented as luminescence (RLU). The graph compared each RNA tested at both growth phases. β-Galactosidase is a commonly used reporter molecule. The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector. Printed in USA. Revised 7/06. The minimum concentration of bovine serum albumin (BSA) read by the GloMax®-Multi Jr Absorbance Module and the BCA Protein Assay is 5 μg/mL (Figure 1), and the maximum is 2 mg/mL (Figure 2). The Enhanced Protocol requires a 30-minute incubate at 60°C. The Standard Protocol permits either a 30-minute incubation at 37°C or a 2-hour incubation |oce| lsr| sxh| sdi| whj| wdw| ccm| zyt| jje| wcv| wuq| vne| nfk| zyc| wxt| fwc| jni| cpo| uxc| hxd| dxg| kxw| rrf| bkb| kjv| mbw| acj| nnp| yxx| gun| kmu| xse| fts| xum| fnm| zxz| ovg| hby| zkk| pmt| xls| tsi| zzv| qry| ecd| osz| xuu| xei| vbf| pum|