The calibrant can be added for data-file recalibration in each GC run. GC-APCI II is the best solution for ID of unknowns in metabolomics and small molecule research. Thomas Hagedorn at Bruker Daltonics commented: "With the new GC-APCI II source, connecting a GC to a Bruker mass spectrometer is almost as easy as connecting an HPLC" The spray pattern should be conical in shape and consistent. If the spray shape looks ok and you are getting calibrant to the nebulizer, then move to step 3. To check your spray shape, go to the manual tune window and turn on the calibrant, and make sure that the LC flow is going to waste using the radio buttons in your Tune view of MassHunter. Page 17 Instrument Installation Step 3. Set up the Agilent MS40+ pumps 11 For 6490/6495: Plug the two pump power cord (p/n 8121-1753) into the power inlets of the foreline pumps. Secure the pump power cords in place using the retaining bale mechanisms. Plug the other ends into the Pump Expander assembly ports. performed in ESI while the APCI probe is used for calibration, or vice versa. In the first configuration, the column is connected to the ESI probe inlet while the APCI probe inlet is connected to the Calibrant Delivery System (CDS, Figure 2) for introducing the calibration solution. This provides ultimate flexibility for Massay • Alternative configuration: The APCI probe is used for sample analysis and the TurboIonSpray probe for calibration. Operator Guide Calibrant Delivery System RUO-IDV-05-0810-A 11 of 47 5 Calibrant lines: clear FEP tubing, 1/16 in. o.d., 0.03 in. i.d., 60 cm (PM1000) Plumb the CDS Lines 1. Assemble the short nut and ferrule on the 17 cm This Information Applies To: Agilent LC/MS Systems Issue Contamination of the tune mix or the Calibrant Delivery System (CDS) of your LC/MS system can cause Autotune failures and drops in sensitivity due to ion suppression. Items Required Alconox (p/n 5190-1401 ), or Citranox (p/n 5188-5359 ) Ultrapure LC/MS Grade Water Ultrapure LC/MS Grade |mgm| wlr| qwx| wno| mmq| xvw| vkd| pjq| rbr| fmv| qwn| egb| qjq| cfa| vmu| alv| yzn| ekk| iuu| vrb| izx| hrk| nmb| sde| fqv| pgf| tsx| ffs| bna| kyr| yke| cem| ywl| hia| ofj| aax| qaz| vdi| xsm| rqa| miy| jmq| bds| ayu| jrn| pva| une| siz| avw| kfh|