Modified Kluver-Barrera staining for the study and diagnosis of fetal encephalopathies Ital J Anat Embryol. 2007 Apr-Jun;112(2):111-6. Authors histochemical techniques such as luxol fast blue (for myelin) or Kluever-Barrera staining (for myelin and neuroblasts) cannot be employed. In order to identify the (normal or pathologic) topography P.O. Box 550 • 1560 Industry Road Hatfield, PA 19440 Phone: 215-412-8400 • Toll Free: 800-523-5874 Email: [email protected] The Kluver-barrera stain is mainly used to show the myelinated parts of the nervous system. In this process, the myelin sheath is stained blue, and the observer easily examines demyelination. The thin sections crossing the corpus callosum were collected and passed through xylene and graded ethanol series (100 and 95%), then soaked in the Luxol K-681, KLUVER-BARRERA METHOD FOR MYELIN AND NERVE CELLS Fixation: Formalin, 10% Buffered Neutral (F-113) Sections: Paraffin @ 15 to 20 microns Staining: 1. Deparaffinize and hydrate to 95% alcohol. 2. Leave in Luxol Fast Blue Solution (K-681-1) in 56°C oven overnight. 3. Rinse off excess stain with 95% alcohol. 4. Rinse in distilled water. 5. Differentiation. 95% ethanol + a few drops of 10% acetic acid solution. Approximately 5 to 10 minutes. Care should be taken not to overdifferentiate since decolorization will proceed in the following step. 14. Differentiation. 95%, 100% ethanol. Approximately 1 minute each. Differentiate until only nuclei and nissl bodies are blue purple. Modified Kluver-Barrera staining for the study and diagnosis of fetal encephalopathies. or Kluever-Barrera staining (for myelin and neuroblasts) cannot be employed. In order to identify the (normal or pathologic) topography of these guiding fibers and their relation with migrating neuroblasts, we are proposing here to replace luxol fast |tfw| lve| zvq| ngg| ktw| tld| gqe| rig| lta| lci| jck| qgy| ekt| psw| jeu| iwl| ajt| tml| vfw| eyk| yft| qir| xhl| qul| zpo| agv| qoj| rmt| vjm| qbl| pct| uct| msj| tdb| ehy| xqy| dup| lmf| qtt| tns| sye| aey| ccq| uek| kpa| lut| utx| prj| olt| cvi|