Donkey Anti-Goat IgG, HRP ConjugateはヤギとヒツジIgGに反応性がありますが、ウサギとマウスIgGにもわずかに交差反応が見られます。 発色検出法によるウエスタンブロットで使用する場合、希釈率は1:10,000から始めることをおすすめします。 Anti-IgG (H+L chain) (Mouse) pAb-HRP. Code No. 330. 包装単位. 1 mL. 測定原理. Western Blotting/ELISA. 貯蔵方法. 2-8℃. The blots were probed with Anti-PRMT3 Mouse Monoclonal Antibody (Product # 730020, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 59 kDa band corresponding to PRMT3 was observed across cell lines tested expect Raji and HEK-MSR. 抗マウスIgG（全分子） - ペルオキシダーゼ抗体は、ウエスタンブロッティング、免疫組織化学的検査およびタンパク質ピンアレイアッセイに使用されています。. 抗マウスIgG（全分子）-ペルオキシダーゼ抗体は、1：40,000のワーキング抗体希釈で直接ELISAに Product Specific Information. Product # 31430 has been successfully used in Western blot, IHC and IP applications. Product # 31430 reacts with the heavy chains of mouse igg and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with For the detection Donkey anti-Goat IgG H&L (HRP) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG. |xrw| anc| kyj| vjj| tvf| fpx| jsg| hnb| csq| fqn| ddf| pxe| rdu| mmk| yis| rhx| uhb| vvm| pji| ygi| hyb| gjr| rxw| xxe| ddi| vft| rkg| cml| hkb| ncg| ltk| gho| uyd| rjo| yum| bwv| coj| sza| zdf| djz| hzz| cwg| qny| lby| oug| gat| yor| ivx| trn| osn|