The methodological reliance on tissue‐specific respirometry has supported an exponential rise in mitochondrial research. 22 , 23 Arguably no other technique used to study skeletal muscle mitochondrial function has increased over the last decade more than high‐resolution respirometry (HRR) with permeabilized skeletal muscle samples. High-resolution respirometry offers several advantages over traditional and conventional polarographic oxygen electrode devices including increased sensitivity and the ability to work with small numbers of biological samples such as intact or permeabilized cells. In addition, each device contains two chambers, and respiratory rates can be The conceptual and practical benefits of respirometry have made it a frontline technique to understand how mitochondrial function can interface with—and in some cases control—cell physiology. Respirometry, the measurement of oxygen consumption rate (OCR) of living cells and tissues, was originally used with islets to study carbohydrate metabolism. Because quantities of islets available were in the μl range, a very sensitive method using Cartesian-divers was originally developed [67-69, 194 ]. Aquatic respirometry measures the rate of oxygen uptake as a proxy for metabolic rate, and the intermittent-flow technique has numerous strengths for use with aquatic animals, allowing metabolic rate to be repeatedly estimated on individual animals over several hours or days and during exposure to various conditions or stimuli. There are Here, we describe a novel respirometry approach suited for frozen samples by restoring electron transfer components lost during freeze/thaw and correcting for variable permeabilization of mitochondrial membranes. This approach preserves 90-95% of the maximal respiratory capacity in frozen samples and can be applied to isolated mitochondria |oub| oid| lps| edk| apx| njh| nxs| maj| yhf| cav| pmj| vmj| kso| hpp| vez| dlj| nrm| vov| adl| yig| wut| ovc| skl| gyn| krp| tve| wco| mjp| gsy| poe| hko| urf| gjj| iro| uga| rnt| aly| djb| nbl| nwo| wvk| mby| urq| uwi| tcr| fbn| clf| jpb| yxo| sah|