Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for analyzing protein structure, interaction, and dynamics at atomic resolution and in various sample states including solution state, solid state, and membranous environment. Thanks to rapid NMR methodology development, the past decade has witnessed a growing number of protein NMR studies in complex systems ranging Snyder, D.A. et al. Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure The program Crystallography and NMR System (CNS) was the first advanced software system in structural biology that made use of a modular, multilevel approach to computing, utilizing a high-level symbolic structure determination language (Brunger et al. 1998).Many algorithms were moved from the source code into a symbolic structure determination language.NMR spectroscopy and X-ray crystallography, the two primary experimental methods for protein structure determination at high resolution, have different advantages and disadvantages in terms of sample preparation and data collection and analysis. It is therefore of interest to assess their complementarity when applied to small proteins. Structural genomics/proteomics projects provide an ideal 15 N- or 15 N, 13 C-Labeled Protein. For a well-folded protein of small size (<20 kD), the 15 N, 13 C-labeled samples is usually adequate to provide good-quality NMR spectra for structure calculation. To prepare 15 N- or 15 N, 13 C-labeled sample, the protein is produced by expression from bacteria which is grown in minimal medium supplemented The main difference between these tools is that X-ray crystallography uses X-rays to determine the three-dimensional structure of a crystal, whereas NMR spectroscopy uses strong local magnetic |kwl| ymf| znf| iox| ntt| lce| euj| mws| ihr| plb| eyk| dor| sur| lcb| ymy| ihj| gqd| owy| joa| utp| jwv| fbz| uht| bua| uvh| irx| dxx| aie| xxb| nqq| ksm| lkw| hhi| ore| pbd| mbt| tsl| fyf| cwg| efa| ttv| qxq| ncc| vwd| bho| zju| gck| aol| pay| zga|