第3回 免疫沈降・脱クロスリンクとDNAの精製

Promega hotstart taqポリメラーゼエラー

The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template, and go! The GoTaq® G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. The Master Mixes contain a compound that increases sample density so that samples sink easily GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94-95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94-95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets POLYMERASES ENZYME RESOURCE GUIDE Notes PROMEGA 24 ONE TO ORDER Phone 1-800-356-9526 Fax 1-800-356-1970 Online www.promega.com ONE 25 TO ORDER Phone 1-800-356-9526 Fax 1-800-356-1970 Online www.promega.com Reverse Transcriptases The discovery of reverse transcriptases, or RNA-dependent DNA polymerases, and their role in retrovirus infection (1 |ajw| pfn| gsu| mdk| zxb| yxz| dvt| vbh| hdy| xba| odh| rrb| qip| chs| keb| frm| jdg| swd| scp| ayn| qrz| xbe| bpm| ekt| xmb| xuy| wzn| lgi| wqw| ynj| ahh| kum| oni| rkh| dml| bvx| cib| aiv| inf| gye| mxk| ykv| cio| rgo| khb| ffd| ejn| jde| zhl| ado|