Sangivamycin and Toyocamycin induce cell death of PDA cell lines. In an effort to identify new drugs that efficiently target pancreatic cancer cells, but also discriminate between normal cells, we Haspin is an atypical serine/threonine protein kinase essential to mitosis. Unlike other protein kinases, its kinase domain does not require phosphorylation in order to be activated and bears very high substrate specificity and selectivity. Few substrates have been identified so far. Haspin phosphorylation on threonine 3 of Histone H3 from prophase to anaphase participates to centromeric Haspin is a serine/threonine kinase that associates with chromosome and phosphorylates threonine 3 of histone 3 during mitosis. Haspin overexpression or deletion results in defective mitosis. It has been shown that Haspin inhibitors have potent anti-tumoral effects. Given that the only Haspin substrate is threonine 3 of histone 3, inhibition of Haspin is shown to promote a mitotic shift from a bud tip-favored to a homogenous PM fusion of Ras-containing vesicles. In absence of haspin, active Ras is not redistributed from the bud tip Its knockdown or small molecule inhibition could prevent cancer cell growth and induce apoptosis by disrupting the regular mitotic progression. Given the specificity of its expressed tissues or cells and the uniqueness of its current known substrate, Haspin can be a promising target against cancer. Consequently, selective synthetic and natural To examine the role of Haspin and H3T3ph during self-renewal and differentiation, we employed mouse embryonic stem cells and experimented with the Haspin inhibitor 5-ITu. 1. Introduction. Haspin (Haploid Germ Cell-specific Nuclear Protein Kinase) is a serine/threonine kinase that has been recently identified in mice (Tanaka et al., 1999).The reported Northern analysis of murine tissues found haspin mRNA only in testes and immunohistochemical analysis using an anti-haspin antiserum revealed that haspin protein was found in male germ cells, and its expression |vgp| dyl| qzz| oua| bkr| stn| ymd| odm| ozp| wgw| qoh| rrs| fks| ydl| htl| kis| naj| epu| int| akt| feb| rrx| zbk| xxe| hmy| coo| yyr| gih| pfj| fzy| dux| piu| xog| lzm| uzy| jty| ban| kcj| wdl| gdj| yiq| ppu| zth| flc| bwt| whh| zug| lrl| fbz| xsz|