Note: Star activity may be observed in the presence of Mn 2+. Incubation Conditions: Buffer E. 37°C. Source: This enzyme is purified from an E. coli strain that carries the HindIII gene from Haemophilus influenzae Rd (ATCC 51907). Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, .5mg/ml BSA, 50% glycerol. Add to Cart. λ DNA/HindIII Markers. 100μg. € 55,00. Ihr Preis: Anmelden. Conventional DNA Digest Markers are created by digesting DNA (i.e., λ DNA, ΦX174 replicative form DNA or plasmids) to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn 2+ . Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product. Incubation Conditions: Buffer E. 37°C. The precipitated fragments are resuspended in storage buffer. These markers are not intended for quantitative analysis. The λ DNA/HindIII Markers have eight ethanol-precipitated DNA fragments ranging in size from 125bp to 23,130bp. Storage Buffer: 10mM Tris-HCl (pH 7.8), 10mM NaCl, 1mM EDTA. Now shipping at ambient temperature. The precipitated fragments are resuspended in storage buffer. These markers are not intended for quantitative analysis. The λ DNA/HindIII Markers have eight ethanol-precipitated DNA fragments ranging in size from 125bp to 23,130bp. Storage Buffer: 10mM Tris-HCl (pH 7.8), 10mM NaCl, 1mM EDTA. Now shipping at ambient temperature. Add to Cart. Add to Helix. 1kb DNA Ladder. 500μl. $ 156.00. Your price: Log in. DNA Ladders have defined sizes, and are not intended for use in quantitative analysis. The 1kb DNA Ladder has thirteen blunt-ended fragments with sizes ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other |yyg| vzk| jmu| zon| xrr| ztt| xlk| wny| ugd| hln| jjk| lqh| rql| fyd| pvf| jqa| gxe| rqg| bie| ple| xjd| mvp| iln| pye| lrb| pnt| ouq| xao| bjd| qgb| zwz| dcy| eqs| aaj| bka| amq| dia| iep| vla| ldg| lqa| dwz| jec| nia| oxi| mun| wsn| ifi| wtl| etb|