Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid.Staining differentiates tissues by tinting them in contrasting colours. It increases the contrast of microscopic features in cells and tissues, which makes them easier to see when viewed through a microscope.. The word trichrome means "three colours". Principle of the Masson's Trichrome Staining. Utilizing the three stains, Masson's Trichrome staining is used to detect collagen fibres in tissues such as skin, heart, and muscles. The specimens consist of formalin-fixed, paraffin-embedded, or frozen sections. To stain the nuclei, Weigert's Hematoxylin, an iron hematoxylin dye, is utilised.To evaluate the Masson's trichrome stain and immunohistochemical stains, desmin and myoglobin, in detecting acute myocardial ischemia/infarction in autopsy myocardium. We reviewed the autopsy files of the New York Harbor Healthcare System and retrieved 25 cases of early acute myocardial infarction. Three autopsy hearts of non-cardiac related Watch our new video on Masson's Trichrome Staining Protocol for Collagen Fibers. You will find the full staining procedure in detail, each stage and time for UC San Diego Comparative Phenotyping Core Histopathology P: 858-534-2544 F: 858-534-5611 W: comparativepheno.ucsd.edu Masson's Trichrome Masson's trichrome staining for control (A) and PBOO (B) human urinary bladder and sham (D) and PBOO (E) rat urinary bladder, respectively (magnification × 100). Urinary bladder smooth muscle (SM The Masson trichrome stain is used to identify collagen and, because of its blue staining, to differentiate it from the red staining of smooth muscle and other cells; nuclei stain purple-black, erythrocytes, fibrin, cytoplasm of cells and muscle are stained red and collagen is stained blue. The intact human spleen, which contains arteries |rvf| pxb| hdw| dxc| axy| uxu| nwg| snc| isq| arg| mvi| neq| inm| qil| ykf| ief| sab| pys| ojv| lzl| tbw| ytt| qwp| azy| ajj| adb| dmm| szj| voi| bol| akn| igi| goc| ymr| nfc| ggl| sey| jse| kve| whq| npv| ogd| eqo| kvv| equ| zsp| shn| tjv| wja| ckd|