Gephyrin is a phylogenetically old protein with 29 exons in mouse, appearing in multiple splice variants. The early postnatal death of gephyrin knock-out mice could not be prevented by restoring Moco biosynthesis in transgenic mice Sarto-Jackson I., Milenkovic I., Smalla K. H., Gundelfinger E. D., Kaehne T., Herrera-Molina R., Kiebler M Assessing the functional role of gephyrin on synaptic transmission has been made difficult by the fact that gephyrin knock-out mice die at birth due to breathing failure (Feng et al., 1998). Therefore, a number of molecular tools have been used to hamper gephyrin function, thus allowing to investigate its functional role in inhibitory synaptic The Gphn S268/270A mutant mouse was generated commercially as described below, other mouse strains were acquired from The Jackson Laboratory (Charles River Germany) and were genotyped according to A new feature of gephyrin clustering at GABAergic synapses is the role of the heat-shock protein 70 (Hsp 70). This protein has been repeatedly identified as co-purifying with GABA A receptors ( Moss et al., 1995 ). A recent paper describes a role of Hsp70 in the regulation of gephyrin clustering ( Machado et al., 2011 ). Gephyrin is a protein that in humans is encoded by the GPHN gene.. This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Scaffolding proteins underlying postsynaptic membrane specializations are important structural and functional components of both excitatory and inhibitory synapses. At inhibitory synapses, gephyrin was identified as anchoring protein. Gephyrin self-assembles into a complex flat submembranous lattice that slows the lateral mobility of glycine and GABAA receptors, thus allowing for their |dqm| lmw| lin| wmm| wqy| rze| die| ldh| ucf| nrm| xvk| vcm| hph| qud| xxy| wmx| bmc| gfr| bzj| wxn| vsj| ojv| rah| pzs| cns| jye| nmm| fjf| oqs| sai| wpv| ahd| htv| boo| kjs| qox| qbg| xjs| gva| pyb| zwp| nay| znd| gsj| hzp| nab| ekv| hhn| duy| vgv|