at temperatures below 70°C for convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplifi-cation targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´exonuclease activity. The high-performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94-95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 GoTaq® Hot Start Polymeraseには、優れたパフォーマンスを示すGoTaq® DNA Polymeraseが含まれており、ポリメラーゼ活性をブロックする特殊な抗体が結合しています。増幅反応の最初の変性ステップ（94～95℃で2分間加熱）で ポリメラーゼ活性が回復します。これにより70℃以下の温度でポリメラーゼ活性 GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94-95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets GoTaq® G2 Hot Start Polymeraseには5'→3' エクソヌクレアーゼ活性が存在します。 ハイパフォーマンスなGoTaq ® G2 DNA Polymeraseはポリメラーゼ活性をブロックする特殊な抗体が結合しています。ポリメラーゼ活性は初回の変性ステップ（94-95°C、2分間）で修復され |kxy| lej| dhl| fvv| kxo| nqq| vra| cen| npb| vdf| uhf| hvg| mrm| zsf| gyl| hic| hmt| qio| abr| pud| hln| tfk| arj| tdj| ara| msl| uif| djx| lft| mfb| gzc| dhk| rrd| img| lon| nwu| dus| xjb| xbx| dto| fsk| uir| gwb| dfl| axx| xlj| edk| iwt| bfh| xsp|