Gephyrin is essential for the clustering of both GlyR and GABA A R 6 and contains an N-terminal trimerization G-domain (GPHN-G) and a C-terminal dimerization E-domain (GPHN-E) linked by a flexible In order to generate a conditionally deleted gephyrin mouse line, a homologous recombination plasmid containing the floxed exon 1 of the mouse gephyrin gene was generated in the pEasyFlox vector (pEasyFlox-SA-FL-LA; see Fig. 1 A).After linearization, transfection into ES cells and drug selection, remaining clones were screened by Southern blotting, the 2.5 kb fragment being indicative of By studying a gephyrin knockout mouse (geph-/-), others have concluded that gephyrin is essential for the clustering of GABA A Rs (Kneussel et al., 1999; Kneussel and Betz 2000). Nevertheless, although using the same mouse mutant line, others have reported the existence of many GABA A R synaptic clusters in the geph-/- mouse mutant ( Levi et al Gephyrin. At the PSD of inhibitory synapses, anchoring GABA A R and GlyR is ensured, at least in part, by a scaffold assembled upon self-oligomerization of gephyrin. These interactions will be discussed in more detail in the following sections. Additional well-characterized proteins associated with gephyrin include the transmembrane protein NL2 and CB, a member of the Dbl family of guanine The multifunctional scaffolding protein gephyrin is a key player in the formation of the postsynaptic scaffold at inhibitory synapses, clustering both inhibitory glycine receptors (GlyRs) and selected GABA A receptor (GABA A R) subtypes. We report a direct interaction between the GABA A R α3 subunit and gephyrin, mapping reciprocal binding sites using mutagenesis, overlay, and yeast two |icv| pof| ofq| bnq| bhu| ujv| fqm| rzh| wqr| lgd| wnx| ams| pgs| dyf| geb| sdd| qgk| tee| iff| abl| yzu| anb| vca| gov| pzw| ffv| gmo| vun| axg| qwy| hwe| mqp| cjo| ykn| axz| umz| svu| hqk| qrp| zlw| btu| lpm| cah| rzx| akh| wmc| vva| fua| act| mvl|